INFN Genova - NF ChAMBRe
ChAMBRe è l’unica camera di simulazione atmosferica in Italia. Nel panorama internazionale, ChAMBRe si caratterizza per lo studio e caratterizzazione degli aerosol atmosferici, in particolare le componenti carboniose e biogeniche. ChAMBRe è equipaggiata per studiare le proprietà ottiche (scattering ed assorbimento) di aerosol primari (freschi ed invecchiati) e secondari e i fenomeni di interazione tra bioaerosol (batteri in particolare) e le altre componenti atmosferiche, inclusi inquinanti gassosi ed altri aerosol. Una più completa descrizione al sito https://labfisa.ge.infn.it/index.php/chambre.
Strumentazione disponibile:
Gas and aerosol inlets
Bio-aerosol nebulizers (BLAM, COLLISON and SLAG by CH-Technologies)
Monodisperse aerosol generator (TSI 1520)
Vacuum system (down to 10-5 mbar)
Sampling ports for filters, impactors and continuous samplers
3 – SUN Solar Simulator (Xenon arc lamp) coupled with a custom quartz window and equipped with AM1.5G filter
Fiber-optic spectrometer (grating 200 – 1091 nm), Avantes
Gas analyzers (O3, NOx, CO/CO2, SOx, BTX)
Real-time aerosol monitoring (APS,OPS & SMPS- TSI)
Bioaerosol on-line spectrometer: WIBS-NEO
3-λ photo-acoustic system (PAXS, λ =870, 532, 405 nm)
CASS and 7-λ aethalometer
Aurora 4000 Nephelometer
Aerodyne ACMS
Aerosol samplers (13-stage Nano-Moudi rotating cascade impactor for size segregated analysis, Battelle Cascade impactor)
Bio-aerosol-specific samplers (Andersen-type cascade impactor, liquid impingers)
Automatized petri-slides exposer
Soot generator: Miniature Inverted Soot Generator, Argonaut Scientific Corporation.
Gas diluter (eDilpro 230, Dekati)
Energy dispersive X-ray fluorescence spectrometer (ED-XRF) Epsilon 4 by Malvern Panalytical
Ion Chromatography for anions, cations and sugars;
Multi-wavelength optical system (MWAA) for non-destructive determination of Black and Brown Carbon;
Two-wavelength Thermo-optical analyzer of TC/OC/EC (SUNSET lab with home-made upgrades)
Basic biological lab equipment allowing for culture analysis in vitro (isolation, identification, growth) and biochemical tests (e.g. catalase and oxidase): autoclave (Asal mod.760), vortex, centrifuge and microcentrifuge (Eppendorf centrifuge 5417R), water purification system Milli-Q- IQ 7003 (Millipore-Elix), incubator for temperature control Ecocell and Friocell MMM Group, Steril-VBH Compact “microbiological safety” cabinet, Thermo electron corporation steri-cycle HEPA Class 100 incubator;
Light and phase-contrast microscopes (Olympus CK2, Nikon Eclipse E200) for micro-organisms size, shape, colony morphology, characterization;
Optical microscope (Nikon Eclipse TE300) for bacterial detection and live/dead discrimination by epifluorescence with specific dyes and for immunoassay fluorescence to label antigenic bacterial target, fluorescent molecule or enzyme.
Link a sito laboratorio: https://labfisa.ge.infn.it/
Riferimenti bibliografici:
Massabò et al., AMT, 2018, doi: 10.5194/amt-11-5885-2018
Danelli et al., AMT, 2021, doi: 10.5194/amt-14-4461-2021
Vernocchi et al., AMT, 2022, doi:https://doi.org/10.5194/amt-15-2159-2022
Massabò, Prati, et al., in Practical Guide to Atmospheric Simulation Chambers, 2023. doi: 10.1007/978-3-031-22277-1
Crawford et al. Atmosphere, 2023, https://doi.org/10.3390/atmos14081214
Vernocchi et a., AMTD, 2023, https://doi.org/10.5194/egusphere-2023-1580
Contatti
- Paolo Prati: prati at ge.infn.it
- Dario Massabò: massabo at ge.infn.it
- Federico Mazzei: federico.mazzei at ge.infn.it
- Virginia Vernocchi: virginia.vernocchi at ge.infn.it