INFN Genova - NF ChAMBRe

ChAMBRe è l’unica camera di simulazione atmosferica in Italia. Nel panorama internazionale, ChAMBRe si caratterizza per lo studio e caratterizzazione degli aerosol atmosferici, in particolare le componenti carboniose e biogeniche. ChAMBRe è equipaggiata per studiare le proprietà ottiche (scattering ed assorbimento) di aerosol primari (freschi ed invecchiati) e secondari e i fenomeni di interazione tra bioaerosol (batteri in particolare) e le altre componenti atmosferiche, inclusi inquinanti gassosi ed altri aerosol. Una più completa descrizione al sito https://labfisa.ge.infn.it/index.php/chambre.

Strumentazione disponibile:

Gas and aerosol inlets
Bio-aerosol nebulizers (BLAM, COLLISON and SLAG by CH-Technologies)
Monodisperse aerosol generator (TSI 1520)
Vacuum system (down to 10-5 mbar)
Sampling ports for filters, impactors and continuous samplers
3 – SUN Solar Simulator (Xenon arc lamp) coupled with a custom quartz window and equipped with AM1.5G filter
Fiber-optic spectrometer (grating 200 – 1091 nm), Avantes
Gas analyzers (O3, NOx, CO/CO2, SOx, BTX)
Real-time aerosol monitoring (APS,OPS & SMPS- TSI)
Bioaerosol on-line spectrometer: WIBS-NEO
3-λ photo-acoustic system (PAXS, λ =870, 532, 405 nm)
CASS and 7-λ aethalometer
Aurora 4000 Nephelometer
Aerodyne ACMS
Aerosol samplers (13-stage Nano-Moudi rotating cascade impactor for size segregated analysis, Battelle Cascade impactor)
Bio-aerosol-specific samplers (Andersen-type cascade impactor, liquid impingers)
Automatized petri-slides exposer
Soot generator: Miniature Inverted Soot Generator, Argonaut Scientific Corporation.
Gas diluter (eDilpro 230, Dekati)
Energy dispersive X-ray fluorescence spectrometer (ED-XRF) Epsilon 4 by Malvern Panalytical
Ion Chromatography for anions, cations and sugars;
Multi-wavelength optical system (MWAA) for non-destructive determination of Black and Brown Carbon;
Two-wavelength Thermo-optical analyzer of TC/OC/EC (SUNSET lab with home-made upgrades)

Basic biological lab equipment allowing for culture analysis in vitro (isolation, identification, growth) and biochemical tests (e.g. catalase and oxidase):  autoclave (Asal mod.760), vortex, centrifuge and microcentrifuge (Eppendorf centrifuge 5417R), water purification system Milli-Q- IQ 7003 (Millipore-Elix), incubator for temperature control Ecocell and Friocell MMM Group, Steril-VBH Compact “microbiological safety” cabinet, Thermo electron corporation steri-cycle HEPA Class 100 incubator;

Light and phase-contrast microscopes (Olympus CK2, Nikon Eclipse E200) for micro-organisms size, shape, colony morphology, characterization;

Optical microscope (Nikon Eclipse TE300) for bacterial detection and live/dead discrimination by epifluorescence with specific dyes and for immunoassay fluorescence to label antigenic bacterial target, fluorescent molecule or enzyme.

Link a sito laboratorio: https://labfisa.ge.infn.it/

Riferimenti bibliografici:

Massabò et al., AMT, 2018, doi: 10.5194/amt-11-5885-2018

Danelli et al., AMT, 2021, doi: 10.5194/amt-14-4461-2021

Vernocchi et al., AMT, 2022, doi:https://doi.org/10.5194/amt-15-2159-2022

Massabò, Prati, et al., in Practical Guide to Atmospheric Simulation Chambers, 2023. doi: 10.1007/978-3-031-22277-1 

Crawford et al. Atmosphere, 2023, https://doi.org/10.3390/atmos14081214

Vernocchi et a., AMTD, 2023, https://doi.org/10.5194/egusphere-2023-1580

The ChAMBRe team
The ChAMBRe team (from left to right) – girls: Elena Gatta, Virginia Vernocchi, Elena Abd El – boys: Muhammad Irfan, Marco Brunoldi, Federico Mazzei, Paolo Prati, Dario Massabò, Tommaso Isolabella, Franco Parodi

Contatti

  • Paolo Prati: prati at ge.infn.it
  • Dario Massabò: massabo at ge.infn.it
  • Federico Mazzei: federico.mazzei at ge.infn.it
  • Virginia Vernocchi: virginia.vernocchi at ge.infn.it

Dove siamo

Vista di insieme di ChAMBRe
Vista di insieme di ChAMBRe
L’interno di ChAMBRe illuminato dalla lampada UV per la sterilizzazione
L’interno di ChAMBRe illuminato dalla lampada UV per la sterilizzazione
Il Solar Simulator sviluppato appositamente per ChAMBRE, potenza massima circa 3 SUN
Il Solar Simulator sviluppato appositamente per ChAMBRE, potenza massima circa 3 SUN
APS (Aerodynamic Particle Sizer, model 3321, TSI Inc.) e WIBS (Waveband Integrated Bioaerosol Sensor (WIBS-NEO, Droplet Measurement Technologies) connessi a ChAMBRe
APS (Aerodynamic Particle Sizer, model 3321, TSI Inc.) e WIBS (Waveband Integrated Bioaerosol Sensor (WIBS-NEO, Droplet Measurement Technologies) connessi a ChAMBRe